Transcriptome-Level Signatures in Gene Expression and Gene Expression Variability during Bacterial Adaptive Evolution.Įrickson, Keesha E Otoupal, Peter B Chatterjee, AnushreeĪntibiotic-resistant bacteria are an increasingly serious public health concern, as strains emerge that demonstrate resistance to almost all available treatments. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. All of these techniques allow efficient and rapid development and screening of bacterial strains having desired phenotypes, and more advanced techniques are expected to be seen. This can be performed by expressing antisense RNA, small regulatory RNA, or CRISPR RNA to repress target gene expression at the transcriptional or translational level. Another approach to achieve desired bacterial phenotypes is the repression of target gene expression without the modification of genome sequences. Due to the increasing demands on high-throughput screening of bacterial strains having the desired phenotypes, several multiplex genome engineering techniques have recently been developed and validated in some bacteria. The group II intron-mediated genome engineering technology is another option for some bacteria that are difficult to be engineered by homologous recombination. ![]() The recombineering technology enables simple and more rapid manipulation of the bacterial genome. During such genome manipulation, the counterselection systems employing SacB or nucleases have mainly been used for the efficient selection of desired engineered strains. Genome engineering has been commonly performed based on homologous recombination. Here we review and discuss the recent advances in bacterial genome manipulation and gene expression control techniques, and their actual uses with accompanying examples. In recent years, a number of techniques and tools have been developed for genome engineering and gene expression control to achieve desired phenotypes of various bacteria. ![]() Song, Chan Woo Lee, Joungmin Lee, Sang Yup Genome engineering and gene expression control for bacterial strain development.
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